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NAD: Shield vs

Browning JD, MacDonald RS, Thornton WH, ODell BL

Some people benefit from weekly injections, while others only need them monthly

Suspend the mince in Solution B and dissociate the tissue by one of the following methods: (a) 500 g papain per brain for 20 min at room temperature followed by DNase I (1 mg/ml) at 37C for 5 min [1] OR (b) 0.25% trypsin and 0.1 mg/ml DNase I for 20 min at 37C [2]