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Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is the gold standard for quantifying dihexa in plasma, with a lower limit of quantification typically around 15 ng/mL depending on sample preparation and ionization efficiency

doi: 10.3389/fimmu.2014.00567 63 LannonSMVanderhoevenJPEschenbachDAGravettMGAdams WaldorfKM

Presuming this is true, we would expect to observe no differences in measured free GSH between the deproteinized heparinized plasma and deproteinized EDTA-treated plasma stored at 80 o C from the same subjects

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