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Signals for protein bands were developed with Super Signal West Femto Chemiluminescent kit (Thermo Fisher Scientific, Waltham, MA) and detected by a Molecular Imager ChemiDoc XRS imaging system (Bio-Rad Laboratories, Hercules, CA)

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HRT is the medical replacement or supplementation of hormones the body has stopped producing adequately most commonly estrogen, progesterone, and testosterone, though thyroid, DHEA, and cortisol may also be part of a comprehensive program