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Determination of initial turnover number The initial turnover rates were determined in PBS at pH 7.4 (8 g l 1 NaCl (Labkem, SOCH-00T), 1.44 g l 1 Na 2 HPO 4 (Supelco, 1.06586), 0.2 g l 1 KCl (Panreac, 131494) and 0.24 g l 1 KH 2 PO 4 (Fisher Scientific, BP362)) using ABTS (1 mM), DMP (5 mM), guaiacol (50 mM), sinapic acid (0.35 mM) or p -coumaric acid (0.1 mM) as substrate

Proline synthesis in Escherichia coli

For single particle analysis, a total of 23,615 micrographs were collected (Supplementary Fig

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Avoiding binge drinkingdefined as five or more drinks in about two hours for men, or four for womenis crucial because rapid, excessive consumption can overwhelm the liver, leading to toxic BAC levels exceeding 0.40%