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Briefly, A549 and THP-1 cells grown on coverslips were incubated with DMSO (vehicle control) or 0.5 M 25HC for 2 h

Pericyte TGF-B signaling supports the BBB integrity by promoting fibronectin expression, basal membrane synthesis, and stimulating tight junction protein expression [34]

Media with no serum enabled stable co-cultures of iPSC-derived microglia and brain endothelial cells which respond appropriately to inflammatory stimuli, such as lipopolysaccharide (Bull et al., Induced Pluripotent Stem Cells Induced pluripotent stem cells have been used in multiple configurations: static transwell mono- or co-cultures (Stebbins et al., 2019) and monoculture microfluidic models (Faley et al., 2019), co-culture with cell lines (Park et al., 2019) or with iPSC-derived neuron or pericyte (Jamieson et al., 2019

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