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Bradnam LV, Graetz LJ, McDonnell MN, Ridding MC

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While they use the same active ingredient, equivalence to FDA-approved finished products should not be assumed

INS-1 832/3 were maintained in RPMI-1640 at 11 mM d-glucose, supplemented with 10% fetal bovine serum (FBS), 10 mM HEPES, 2 mM l-glutamine, 1 mM pyruvate, 50 M -mercaptoethanol, and 1% penicillin/streptomycin 21

N-ethylmaleimide is frequently used to trap free thiols, but reagent choice, timing, collection tubes, anticoagulants, storage conditions, and freeze-thaw limits must be validated for the matrix and analytical method