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Nonmigrated cells were removed with cotton swabs, and migrated cells were fixed with ice-cold methanol and stained with 4,6-diamidino-2-phenylindole (DAPI)

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In light of this, our finding points out that during the period of retinal maturation when there are significant structural maintenance demands requiring elevated lipid and protein synthesis, a careful balance needs to be maintained in the use of NADPH between antioxidant metabolism and biosynthetic processes