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RNA isolation and qRT-PCR Total RNA was extracted from primary astrocytes and striatum tissue using TRIzol reagent (Invitrogen, 15596026) and reversed transcribed into cDNA using PrimeScript RT Master Mix (Takara, RR036A)

The potential of fertirelin extends beyond the current applications

In vitro experiments further confirmed that Que significantly upregulated AMPK expression in oleic acid (OA)-stimulated hepatocytes, increased PINK1 and Parkin protein levels, and enhanced mitophagy, which subsequently inhibited NLRP3 and IL-1 expression, downregulated ROS production, and attenuated OA-induced hepatocyte injury ( In summary, enhanced mitophagy can reduce hepatic inflammation, OS, apoptosis, lipid accumulation, and steatosis, and inhibit the developmental process of MAFLD

Consistent timing produces consistent tissue concentrations and more reliable results