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The lysates were cleared at 8500 x g for 20 min at 4 C and incubated at 4 C for 3 h with 100 L of Glutathione Sepharose 4B (Cytiva)

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The first two bind to the xenobiotic response element (XRE) sequences, while the last binds to the NF-B binding site in the NFE2L2 promoter, inducing its transcription [171,172,173,174], with a consequent increase of NFE2L2 mRNA available within the cell