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The reconstitution math above reflects community-documented protocols, not instructions from this site

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Medical Disclaimer: The information provided in this article is for educational and reference purposes only and does not constitute medical advice or be used as a substitute for professional medical diagnosis, treatment, or advice

Immunofluorescence microscopy Cells were grown on glass coverslips and fixed with 4% (w/v) paraformaldehyde (PFA) in PBS for 20 min at room temperature and permeabilized with 0.2% (w/v) saponin in PBS for 5 min at room temperature