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On the day of the assay, serial dilutions (7-fold dilutions, 7 concentrations per compound and one well containing only assay buffer) of test compounds were performed in duplicate in assay buffer to appropriate concentrations in 96-well plates (Greiner, U-shape #GR-650201) using a Biomek i7 liquid handler

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doi: 10.1186/1476-9255-10-12 [DOI] [PMC free article] [PubMed] [Google Scholar] 107.Behnen M, Leschczyk C, Mller S, et al