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The homogenate was centrifuged at 300 g for 10 minutes at 25C, after which the upper phase was discarded

These plasmids were isolated from bacteria using a HiSpeed Midi Kit (Qiagen, Valencia, CA)

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Dihexa or its analogs were administered 5 minutes after scopolamine, either intracerebroventricularly at doses of 0.1 or 1 nmol), intraperitoneally at doses of 0.05, 0.25, or 0.50 mg/kg, or orally at doses of 1.25 or 2.0 mg/kg