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For actin and Drebrin immunoblots, proteins were prepared in NuPAGE LDS sample buffer (4) (Life Technologies, NP0007), split into reduced (+2% 2-ME) and nonreduced aliquots and heated at 70 C for 10 min 28

In some embodiments, a high viscosity otic formulation or device described herein provides an apparent viscosity of from about 100,000 cP to about 1,000,000 cP